Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols ((send by free-web-host.me user))
COLD SPRING HARBOR, N.Y. (Wed., Aug. 5, 2009) â€" Vectors derived from retroviruses are useful tools for long-term gene transfer because they allow stable integration of transgenes and propagation into daughter cells. Lentiviral vectors are preferred because they can transduce non-proliferating cellular targets. These vectors can be engineered to target specific tissues. In the August issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc8_09.dtl), François-Loïc Cosset and colleagues from Ecole Normale Supérieure de Lyon (http://hvd.ens-lyon.fr/human_virology_dpt) present a method for targeting hematopoietic stem cells using engineered viral vectors. The article, "Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors," is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/8/pdb.prot5276).
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